Description:

TheTECO®MucusCollectionSetisacollectionsetforfishmucus,includingvalidatedswabs,sampletubesinasampletuberack,aspecificallydesignedextractionbufferandakitinstruction.TheMucusCollectionSetisavalidatedtoolforrepeatablenon-invasive/non-destructivesamplingofepidermalmucosaforvitellogendetermination.

TheTECO®MucusCollectionSetforfishmucusisavalidatedtoolforrepeatablenon-destructivesamplingofepidermalmucosaforvitellogenindeterminationusingthe:

• TECO®Perch(Perciformes)VitellogeninELISAKit,TE1035
• TECO®CyprinidVitellogeninELISAKit,TE1037
  Componentsarereadytouse.

KitComposition:

1: BreakableSwabs,42. Readytouse.
2: ReactionVials,42. Readytouse.
3: ExtractionBuffer,1x25ml Readytouse.
4: RackforReactionVials,1x
I:Kitinstruction,1x

Background:

Vitellogenin(VTG)isoneofthecoreendpointsinscreeningandtestingforendocrinedisruptingchemicalsstandardizedintheOECDGuidelinesforthetestingofchemicalsforestrogenicactivity.Originallybelievedtobeproducedonlyintheliver,severalcelltypeshaverecentlybeenshowntoproduceVTGafterestrogenstimulation,includingthoseoftheepidermalmucosaoffish.

GiventhedestructivenatureoftrADItionalsampling,collectingsuccessivesamplesfromfishhasbeenawidespreadproblem,asforinstanceintheanalysisofvitellogenininductionwhilescreeningandtestingchemicalsubstancesforendocrinedisruptionpotential.Bloodisdifficulttocollect,inparticularwhereverysmallfishareconcerned,orinapproacheswheretheanimalsmustsurvivesampling.Thisisparticularlyimportantinfieldmonitoringinordertoavoidimpactonthepopulationunderinvestigation.

EventhoughtheVTGconcentrationintheskinmucusisanorderofmagnitudelowerthaninbloodserumorinbodyhomogenates(containinglivertissue),theskinmucosaisverywellsuitedasamatrixtodetermineexogenousVTGinductioncausedbyenvironmentalchemicalswithaffinitytotheestrogenreceptors.Mucussamplingwiththeaidofswabsconstitutesanalternativetotraditionalprocedures,avoidinganimalinjurybyusingahighlysensitiveELISAincombinationwithahighqualityextractionbufferandswabsampling,thedeterminationofmucosa-derivedVTGhasthefollowingadvantages:

• Non-destructive,non-invasivesampling
• Definedmatrixwithoutlymphaticfluidcontamination
• Allowstheuseofsmallerfishfortestingandmonitoringpurposes
• Repeatedsamplingallowsforindividualrecordingoftheinductionkinetic

Thesamplingtechniqueleadstohighlycomparableresultswhentestingsamplesfromtheleftandtherightsidefromthesameindividualininter-laboratorytrials.