Description:

DISCONTINUED

Multiplate®PAR-4testisareagentkitforuseinplatelet aggregationstudiesontheMultiplate®analyzerto evaluatethequalitativeplateletfunctioninspecies expressingPAR-4receptors.InparticularPAR-4testcan beusedfordeterminationofPAR-mediatedaggregation inspeciesthatdonotexpressPAR-1(stimulatedby TRAP-6).

ThrombinisoneofthemostpotentplateletagoNISTs.It interactswith2protease-activatedreceptors(PARs)on thesurfaceofhumanplatelets–PAR-1andPAR-4.The expressionprofilesofPARsonplateletsdifferbetween humansandnonprimates.PAR-1isbelievedtobethe primaryreceptorforthrombininhumans.Incontrast, mouseplateletsforexamplelackPAR-1andlargely signalthroughPAR-4inresponsetothrombin.

KitComposition:

Reagents,StorageandStABIlity

• MP0549:PAR-4test,PAR-4Agonist, 1x1.0ml,lyophilized(20mM)with5microtesttubesfor aliquotation. PAR-4testreagent isstableuntiltheexpirydateprinted ontheviallabelwhenstoredat 2-8°C, 14daysafterreconstitutionwhenstoredat 2-8°C,4weekswhen storedat<-20°Cor24hoursafterthawing.If reconstitutedreagentisnottransferredintomicrotest tubes,theoriginalvialshouldbestoredinanupright position.

MeasurementPrinciple:

PAR-1isbelievedtobetheprimaryplateletreceptorforthrombininhumans.Thisreceptorisactivatedby TRAP-6inMultiplate.Incontrasttohumans,severalanimalspecies(e.g.,mice,dogs)lackPAR-1receptorsandlargelysignalthroughPAR-4inresponsetothrombin.Eveninhumans,plateletPAR-4receptorscanbestimulatedtotriggerplateletactivationandaggregation.InordertoreachasimilarextentofaggregationaswiththeTRAPtest,significantlyhigherconcentrationsofPAR-4agonisthavetobeapplied(645µMforPAR-4comparedto32µMforTRAP-6).