Description:

DISCONTINUED–REPLACEMENT:MP0260

Multiplate®ProstaglandinE1(PGE1)isareagentkitfor theassessmentofADPtestHSand assessmentofpositive(i.e.abnormal) controlsoftheADPtest ontheMultiplate®instrument. Fortheevaluationofqualitativeplateletdefectsaswellas plateletfunctioninhibition.PGE1enhancesthesensitivity ofADPtesttoplateletfunctioninhibitionespeciallytothe effectofclopidogrel.Moreover,theadditionofahigher doseofPGE1intothesamplecaninduceanabnormal aggregationinADPtest,whichallowstheperformanceof anabnormalcontrol(positivecontrol).ProstaglandinE1(PGE1)isusedincombinationwiththe ADPtestreagent(MP0120).IntheADPtestHS,anincompleteblockadeofaggregationisoftenseenwithclopidogreltherapy.ThismaybeduetothefactthatADPnotonlytriggerstheP2Y12 receptorontheplateletsurface(e.g.,thereceptorthatisblockedbyclopidogrel),butalsootherADPreceptors(whicharenotaffectedbycopidogrel).AcombinationofADPandphysiologicalplateletinhibitor(prostaglandinE1(PGE1))canbemoresensitiveforthedetectionoftheactionofclopidogrelthantheuseofADPalone.ThebindingofADPtotheP2Y12receptorreducesthelevelofcAMPintheplatelet,whichinturnenhancesthereleaseofcalciumfromendogenoussources.Theenhancementofintracellularcalciumleadstotheactivationandaggregationoftheplatelet.PGE1reducesthemobilizationofcalciumandthusinhibitsplateletaggregation.AsclopidogrelalsoreducestheplateletactivationbyADP,clopidogrelandPGE1actsynergistically.

KitComposition:

Reagents,StorageandStABIlity

• MP0160:Multiplate®ProstaglandinE1, PGE1,Kit,1x1.0mL, lyophilized(300nM),with5microtesttubesfor aliquotation. An unopenedreagentisstableuntiltheexpirydateprinted ontheviallabelwhenstoredat 2-8°C, 7daysafterreconstitutionwhenstoredat 2-8°C,4weeks <-20°C,or 24hoursatroomtemperatureafterone timethawing.

AlsoAvailable

• MP0096: Multiplate®PGtestAliquotVials,Bag,100microtubescolorcodedmicrotesttubesfor aliquotation.
• MP0260:Multiplate®ProstaglandinE1, PGE1, Kit,3x1.0mL, lyophilized(300nM),withoutmicrotesttubesfor aliquotation.
• MP0540:Multiplate®ProstaglandinE1, PGE1, Bag,1x1.0mL,lyophilized(300nM),withoutmicrotesttubesfor aliquotation.

MeasurementPrinciple:

PGE1isanaturalplateletinhibitorwhichtriggersan increaseincAMPlevelsintheplatelet.cAMPisasocalled secondmessenger,i.e.anintracellularsignalling molecule.AdecreaseofthecAMPlevelintheplatelet leadstoplateletactivation.AnincreaseofthecAMPlevel counteractsplateletactivation. Theadditionof20µlPGE1totheADPtest(9.4nMPGE1 finalconc.)inducesamoderateinhibitionofplatelet activationinhealthynormalbloodsamples,buta significantincreaseofsensitivityoftheADPtesttothe plateletinhibitionbyclopidogrel. Theadditionof50µlPGE1intoADPtest(22nMPGE1 finalconc.)normallyinducesastronginhibitionofADP inducedaggregation(positivecontrolforADPtest).