Description:
ForusewithMultiplate®
Multiplate®HirudinBloodTube(Double-Wall)isabloodcollectiontubeforuseinplatelet aggregationstudieswiththeMultiplate®plateletfunction analyzertoevaluatequalitativeplateletdisordersat physiologicalcalciumconditions. Hirudin,athrombininhibitorallowsanticoagulationof bloodwithoutinterferencewithphysiologicalcalcium levels.
DuringtheearlyphaseofMultiplate®development,citratedblood(either3.2%or3.8%)was usedforthesamplecollection. Itwasobservedthatsubjectsdidnotaggregateproperly,particularlywithADPasagoNIST.Citratecomplexescalciumandtherebypreventsclottingofthesample.However,calciumisalsoanimportantsecondmessengerforplateletaggregation.Forafullshapechangeandfullexposureofreceptorsontheplateletsurface,thereleaseofcalciumfromtheintracellularstoresisnecessary.Thebestsolutionforthisproblemistouseananticoagulantwhichdoesnotaffectthefreecalciumconcentrationinthesample, ideallyhirudin(adirectthrombininhibitor).Heparinhassomeactivatingpropertiesonplatelets;therefore,theuseofhirudinbloodispreferred.Toreducetheinfluenceofcalciumdepletionbycitrate,apartialrecalcificationusingtheNaCl/CaCl2diluentsolution isused.ThepartialrecalcificationcanleadtoadisaggregationphenomenonintheASPItestandRISTOtest.Forthesetests,0.9% salineisusedwhentesting citratedblood.
Pleasenotethecatalog#forMultiplateHirudinVacuumBloodCollectionTubeshaschangedto08128812001.Youmayhavepreviouslyorderedthisproductundercatalog#MP0600or06675751001.
ForusewithT-TAS®plus
Composition:
Reagents,StorageandStABIlity
- 08128812001: Multiplate®HirudinBloodTube(Double-Wall),50x3.0mltubes,anticoagulant:recombinanthirudin
Nolongeravailable
- MP0601: Multiplate®HirudinBloodTube(Double-Wall),10x3.0mltubes,anticoagulant:recombinanthirudin
- MP0631: Multiplate®TI–ThrombinInhibitorBloodCollectionTube, 10x1.25mlmicrotubeswithscrewcap,anticoagulant:recombinanthirudin
Thespecifiedconcentrationofhirudinintheblood collectiontubesis>15µg/ml.Storetubesat4-25°C.Avoidexposuretodirectsunlight.
MeasurementPrinciple:
Refertopackageinsert.
