Description:

DISCONTINUED–REPLACEMENT:MP0240

Multiplate®RISTOtestisaresearchreagentkitforthequantitativeinvitrodeterminationofvonWillebrandFactor(vWF)-andglycoproteinIb(GpIb)-dependentplateletaggregationstudiesontheMultiplate®analyzer.

RistocetinisanantimicrobialsubstancewhichformscomplexeswithvWF(vonWillebrandfactor).Inthiscomplex,vWFchangesitsconformationinawaythatallowsittobindtoplatelets.Thebindingofristocetin-vWFcomplexesleadstotheaggregationandactivationofplatelets.ThisactivationreliesonthebindingofvWFtotheGPIbreceptoranditisinhibitedbytheactionofAspirin®.ThisexplainstheAspirin®sensitivityofRISTOtest.CompletelackofaggregationintheresearchRISTOhightestcanbecausedbyanabsenceofGPIbreceptorsontheplateletsurface(Bernard-Souliersyndrome)orbyanabsenceofvWFinthesolution(vWDtypeIII).

KitComposition:

Reagents,StorageandStABIlity

• MP0140:Multiplate®RISTOtestReagent,Kit,Ristocetin,1x1.0mL, lyophilized(10mg/ml),with5microtesttubesfor aliquotation.UnopenedvialsoftheRISTOtestreagentmustbestored at2-8°C.Thereagentisstableuntiltheexpirydate printedontheviallabelwhenstoredunderthese conditions.Ifreconstitutedreagentisnotaliquotedinto microtesttubes,theoriginalvialshouldbestoredinan uprightposition. Stable7daysafterreconstitutionwhenstoredat 2-8°C, 4weeks at<-20°Cor24hoursatroomtemperatureafterone timethawing.

AlsoAvailable

• MP0094: Multiplate®RISTOtestAliquotVials,Bag, 100microtubescolorcoded(blue)forfor aliquotation.
• MP0194:Multiplate®RISTOtestReagent,Bag, Ristocetin,1x1.0mL, lyophilized(10mg/ml),withoutmicrotesttubesfor aliquotation.
• MP0240:Multiplate®RISTOtestReagent,Kit,Ristocetin,3x1.0mL, lyophilized(10mg/mL),withoutmicrotesttubesfor aliquotation.

MeasurementPrinciple:

RISTOtestreagentcontainsristocetin.Ristocetinformsa complexwithvWFfromthesample.Thiscomplexbinds totheplateletGpIbreceptorandtriggersplatelet activationandaggregation.RISTOtestcanbeappliedin twoconcentrations:InRISTOhightestahigh concentrationofristocetin(0.77mg/mL)isapplied,which normallyinducesastrongplateletaggregation.Abolished aggregationinRISTOhightestcanbebasedona deficiencyofGpIbreceptorsorvWF.InRISTOlowtesta lowerconcentrationofristocetin(0.2mg/mL)isapplied whichnormallydoesnotinduceastrongaggregation response.Ahigherthanexpectedaggregationin RISTOlowtestmayindicateanenhancedaggregation tendencyofvWF(vWDtypeIIb).