Description:

Multiplate®RISTOtestisaresearchreagentkitforthequantitativeinvitrodeterminationofvonWillebrandFactor(vWF)-andglycoproteinIb(GpIb)-dependentplateletaggregationstudiesontheMultiplate®analyzer.

RistocetinisanantimicrobialsubstancewhichformscomplexeswithvWF(vonWillebrandfactor).Inthiscomplex,vWFchangesitsconformationinawaythatallowsittobindtoplatelets.Thebindingofristocetin-vWFcomplexesleadstotheaggregationandactivationofplatelets.ThisactivationreliesonthebindingofvWFtotheGPIbreceptoranditisinhibitedbytheactionofAspirin®.ThisexplainstheAspirin®sensitivityofRISTOtest.CompletelackofaggregationintheresearchRISTOhightestcanbecausedbyanabsenceofGPIbreceptorsontheplateletsurface(Bernard-Souliersyndrome)orbyanabsenceofvWFinthesolution(vWDtypeIII).

Pleasenotethecatalog#forMultiplate®RISTOtestKithaschangedto08116644001.Youmayhavepreviouslyorderedthisproductundercatalog#sMP0240or00675867190.

KitComposition:

Reagents,StorageandStABIlity

• 08116644001:Multiplate®RISTOtestReagent,Kit,Ristocetin,3x1.0ml, lyophilized(10mg/ml),withoutmicrotesttubesfor aliquotation. UnopenedvialsoftheRISTOtestreagentmustbestored at2-8°C.Thereagentisstableuntiltheexpirydate printedontheviallabelwhenstoredunderthese conditions.Ifreconstitutedreagentisnotaliquotedinto microtesttubes,theoriginalvialshouldbestoredinan uprightposition. Stable7daysafterreconstitutionwhenstoredat 2-8°C, 4weeks at<-20°Cor24hoursatroomtemperatureafterone timethawing.

AlsoAvailable

• 06675727190: Multiplate®RISTOtestAliquotVials,Box, 100microtubescolorcoded(blue)forfor aliquotation.

Nolongeravailable

• MP0140:Multiplate®RISTOtestReagent,Kit,Ristocetin,1x1.0ml, lyophilized(10mg/ml),with5microtesttubesfor aliquotation.
• MP0194:Multiplate®RISTOtestReagent,Bag, Ristocetin,1x1.0ml, lyophilized(10mg/ml),withoutmicrotesttubesfor aliquotation.

MeasurementPrinciple:

RISTOtestreagentcontainsristocetin.Ristocetinformsa complexwithvWFfromthesample.Thiscomplexbinds totheplateletGpIbreceptorandtriggersplatelet activationandaggregation.RISTOtestcanbeappliedin twoconcentrations:InRISTOhightestahigh concentrationofristocetin(0.77mg/ml)isapplied,which normallyinducesastrongplateletaggregation.Abolished aggregationinRISTOhightestcanbebasedona deficiencyofGpIbreceptorsorvWF.InRISTOlowtesta lowerconcentrationofristocetin(0.2mg/ml)isapplied whichnormallydoesnotinduceastrongaggregation response.Ahigherthanexpectedaggregationin RISTOlowtestmayindicateanenhancedaggregation tendencyofvWF(vWDtypeIIb).