Description:
Multiplate®COLtestisareagentkit usedforcollageninducedaggregation inresearchof plateletaggregationstudiesfortheevaluationofnormalplateletfunctionontheMultiplate®Analyzer. Sensitivetoacyclooxygenaseinhibition,GpIIb/IIIa antagoNISTsanddeficiencyofGpIIb/IIIareceptors. Fortheresearch ofqualitativeplateletfunctioninhibition.
Pleasenotethecatalog#forMultiplate®COLtestKithaschangedto08115842001.Youmayhavepreviouslyorderedthisproductundercatalog#sMP0230,06675832190,08115842001DorDPGAG005K.
KitComposition:
Reagents,Composition,StorageandStABIlity
- 08115842001:Multiplate®COLtestreagent, Kit,3x1.0mL.150tests. Reagentisstableuntiltheexpirationdateprintedonthe viallabelwhenstoredat2-8°C. Stable7daysafterreconstitution.Donotfreezethereconstitutedreagent.
Alsoavailable
- 06675697190: Multiplate®COLtestAliquotVials,Box, 100 colorcoded(white)microtesttubes.
Nolongeravailable
- MP0193:Multiplate®COLtestreagent,Kit,1x1.0mL.50tests
- MP0130:Multiplate®COLtestreagent,Bag, 1x1.0mL,5colorcoded(white)microtesttubesfor aliquotation.50tests.
MeasurementPrinciple:
TheCOLtestleadstoplateletactivationviatheplateletcollagenreceptor.Forsuitableplateletactivation,thereleaseofendogenousarachidonicacidfromtheplateletphospholipidsisnecessary.ThearachidonicacidisthentransformedtotheplateletagonistthromboxaneA2(TXA2)bytheenzymecyclooxygenase.CyclooxygenaseisblockedbyAspirin®.Therefore,theCOLtestissensitivetoAspirin®action.ThismechanismalsoexplainswhytheCOLtestislessspecifictowardstheactionofAspirin®ascomparedtotheASPItest.IntheASPItest,adefinedamountofarachidonicacidisusedastheactivator.However,inCOLtest,plateletsareactivatedthroughendogenousTXA2andotherTXA2independentmechanisms.
