Description:
Multiplate® analysistakesplaceinasingle-usetestcellwhichincorporatesdualsensorsandateflon-coatedstirringmagnet. AkeyinnovationofMultiplate® istheuseoftheMultipleElectrodeAggregometry(MEA)technology.
TheprincipleofMultiplate® analysisisbasedonthefactthatplateletsbecomestickyuponactivationandadhereandaggregateontothemetalsensorwiresintheMultiplate® testcell.Thetestcellhasapipettinginletandacupportionwiththesensorwireswhichextendintothebloodsample.Thesensorwiresaremadeofhighlyconductivecopperwhicharesilver-coated.Thetestcellisconnectedtotheinstrument usingasensorcableandtheelectricalresistancebetweenthesensorwiresisrecordedduringthetest.Whenactivated,plateletsadheretothesensorwiresandtheelectricalresistancebetweenthewiresincreases.
Features:
- Wholeblood,nosampleprocessing
- Smallsamplevolume(300µl)
- Minitestcells use175µlofwholeblood;smallestvolumerequirementforaggregometrytesting.Idealforpediatricplateletresearchor animalplateletstudies.
- Upto30testsperhour
- Interactiveelectronicpipetting
- 5independentchannelsforparalleltesting
- Specificandsensitivereagents
- Standardizedtestprocedures
- Opensystemforresearchapplications
- Windows-basedsoftware
- Newtestscanbedefinedinthesystemsoftware
What"sIntheBox?:
- 1× Multiplate® 5.0Analyzer(MP0010)
- 1× Multiplate®ElectronicPipette(MP0030)
- 1× Multiplate®PipetteTips(MP0040)
- 1× Multiplate®SensorCables(MP0360)
- 1× Multiplate®TestCells(MP0027)
- 1× Multiplate®ReagentRack(MP0370)
- 1× Multiplate®Keyboard(MP0441-EU)
- 1× Multiplate®Mouse(MP0032)
- 1× LCDmonitorwithhospitalgradepowercord(MP-MONITOR)
- 1× Printer(PRINTER)
- 1× Multiplate®Manual
Reagents:
ThefollowingreagentsareavailableforMultiplate®
- Multiplate®ASPItest(08115826001)
- Multiplate®ADPtest(08115761001)
- Multiplate®COLtest(08115842001)
- Multiplate®RISTOtest(08116644001)
- Multiplate®TRAPtest(08116679001)
- Multiplate®ProstaglandinE1(08115982001)
- Multiplate®GpIIbIIIa-AntagoNIST(08115869001)
TestResults:
TheMultiplate®researchanalyzerrecordsplateletaggregationatapproximately0.5secondintervals.TheincreaseinimpedancebytheattachmentofplateletsontotheMultiplate®sensorsistransformedto aggregationunits(AU)andplottedagainsttime.
Threeparametersarecalculatedwhenperformingresearch;Aggregation,AUC,andvelocity.ThemostimportantparameteristheAreaUndertheaggregationCurve(AUC).AUCisrecordedasUnitsorU.Itisaffectedbythetotalheightoftheaggregationcurveaswellasbyitsslopeandisbestsuitedtoexpresstheoverallplateletactivity.TheAggregation(inAU)isthemaximumheightofthecurveduringthemeasurementperiodandtheVelocity(inAU/min)isthemaximumslopeofthecurve.
Theoutputdatacalculatedbythesoftwareisthemeanvalueofthetwoindependentsensorsinthetestcell.Thecorrelationcoefficientoftheindividualmeasurementsisdeterminedandtheanalysisisacceptedwhenthecorrelationcoefficientisgreaterthan0.98.Additionally,thedifferenceofeachcurvefromthemeancurve(DIF)iscalculatedandisacceptedwhenthedifferenceislessthan20%.
[Note:AnearlierversionofthesoftwareexpressedAUCasAU*min.They-axisisaggregationinaggregationunits(AU)andthex-axisisthetimeinminutes.Toconverttocurrentterminology,10AU*min=1U.
Publications:
ComparisonofplateletaggregationusinglighttransmissionandmultipleelectrodeaggregometryinGlanzmannthrombasthenia.
AwidiA,MaqablahA,DweikM,BsoulN,Abu-KhaderA.
Platelets.Aug2009;20(5):297-301.
Assessmentofplateletfunctiononwholebloodbymultipleelectrodeaggregometryinhigh-riskpatientswithcoronaryarterydiseasereceivingantiplatelettherapy.
PanicciaR,AntonucciE,MagginiN,RomanoE,GoriAM,MarcucciR,PriscoD,AbbateR.
AmJClinPathol.2009;131:834-842.
Inhibitionofplateletfunctionwithclopidogrel,asmeasuredwithanovelwholebloodimpedanceaggregometerinhorses.
RoscherKA,FailingK,MoritzA.
TheVetJ.2015;203:332-336.
Multipleelectrodeaggregometry:Anewdevicetomeasureplateletaggregationinwholeblood.
TóthO,CalatzisA,PenzS,LosonczyH,SiessW.
ThrombHaemost.2006;96:781-788.
