Description:
TheTECO®UrinaryCollagenIVELISAprovidesamethodforthequantitativedeterminationofcollagenIVinhumanurine.
KitComposition:
- CollagenIVAntibodycoatedMicroassayPlate12stripsof8wells(96beakapartwellsintotal),inaframe.ReadytoUse
- CollagenIVCalibrator—StandardAReadytoUse,0.8ug/L
- CollagenIVCalibrator—StandardBReadytoUse,3.2ug/L
- CollagenIVCalibrator—StandardCReadytoUse,12.5ug/L
- CollagenIVCalibrator—StandardDReadytoUse,50ug/L
- 10XWashBufferConcentrate
- AssayBufferReadytoUse
- EnzymeConjugateAnti-collagenIFmouseFab’conjugatedtoHRP.ReadytoUse
- TMBSubstrateReadytoUse
- StopSolution1MSulphuricAcid,ReadytoUse
- PlateSeal
AssayPrinciple:
TheTECO®UrinaryCollagenIVELISAisdesignedfortheassayofurinarycollagenIV.Itisasolidphaseone-stepsandwichELISA.CollagenIVinthesampleisboundsimultaneouslybyasolidphasemonoclonalantibodyandamonoclonalantibody-enzymeconjugatedirectedatdifferentantigenicsites.ThisresultsinthecollagenIVmoleculebeingsandwichedbetweenthesolidphaseandenzymelabelledantibodies.Afterremovingunboundenzymelabelledantibodyandsample,theplateisthenincubatedwithenzymesubstrate,resultinginthedevelopmentofacolor.ThecolordevelopedisdirectlyproportionaltotheamountofcollagenIVinthesample.
TypicalCalibrationCurve
TypicalcalibrationcurveobtainedusingSerumCollagenIVELISA.4-ParameterplotofA450/630nmversusug/LCollagenIV.Assayrangeis0.8-50ug/LCollagenIV.
Background:
CollagenIVisatypeofnon-helicalformingcollagenfoundprimarilyisthebasallamina(basementmembrane),anditisconsideredtoconstituteitsbasicframework.UrinarycollagenIVlevelsareelevatedinavarietyofrenalpathologies,particularlydiabeticnephropathy.UrinarycollagenIVissignificantlyhigherinpatientswithnon-insulindependentdiabeticmellitus(NIDDM)thaninnormalsubjectsandurinarycollagenIVlevelscorrelatewiththedepositionofcollagenIVinthekidney.Indiabeticsubjects,urinarycollagenIVissignificantlyincreasedinpatientswithmicroalbuminuriaorovertproteinuriaascomparedtothosewithnormoalbuminuria.Moreover,indiabeticswithnormoalbuminuria,thosewithelevatedurinarycollagenIVwereatanincreasedriskforprogressiontomicroalbuminuria.IntensivetherapyofdiabeticnephropathycanslowthetemporalincreaseinurinarycollagenIVindiabeticsindicatingthepotentialofurinarycollagenIVforstudyingtherenaleffectsofnewtherapies.TheseresultssuggestthatthemeasurementofUrinaryCollagenIVmightprovideausefulbioMarkerforstudyingdiabeticnephropathy.
Inthefieldofrenaltransplantation,renalcollagenIVlevelsareincreasedandincreasedurinarycollagenIVlevelsarefoundinacuterenalrejection,indicatingthepotentialvalueofurinarycollagenIVinstudyingtheseconditions.
