Description:

Technozym®ADAMTS-13Inhibitor ELISAisatestkitfordetectionofhumanautoantibodies(IgG)inserumorplasmaagainstADAMTS-13,theproteasewhichisresponsIBLeforcleavingunusuallylargevWF(ULvWF).MostoftheseautoantibodiesinhibitADAMTS-13activityandthusnon-cleavedULvWFaccumulatesinplasma.TechnozymADAMTS-13INHassay makesitpossibletoresearchthedifferentiatebetweencongenital(genepolymorphisms)andacquired(autoantibodies)whencoupledtoanactivityassay.

KitComposition:

• ELISAteststrips(6),with8wellseach,coatedwitharecombinantformofADAMTS-13protease;thedryingagentissuppliedinanaluminiumbag.
• Washingbufferconcentrate(PBS;pH7.3);containingdetergent;0.01%merthiolate;1vial,100mL.
• Incubationbuffer(=sampledilutionbuffer)(PBS;pH7.3);containsstABIliserprotein;0.01%merthiolate;anddye,1vial,100mL,readyforuse.
• Calibrators(standards)numberedfrom1to5;lyophilised;1vialeach;0.5mL.Concentrationsarelot-specific;consultthelabelonthevial.
• Controlplasma(positiveandnegative);lyophilised;1vialeach;0.5mL.Concentrationsarelot-specific;consultthelabelonthevial.
• Conjugate:anti-humanIgGPOX;dyedblue;1vial,0.3mL.
• ChromogenicsubstrateTMB(tetramethylbenzidine);1vial;12mL;readytouse.
• Stoppingsolutionsulphuricacid1.9mol/L;1vial;12mL;readytouse.
• Adhesivefilm:forELISAteststrips;2pieces.

Advantages:

• Specificforanti-ADAMTS-13IgG
• NofalsepositiveresultswithsamplescontaininghighamountsofIgGingeneral
• Reproducibleandsimple–easytostandardize
• Ready-to-usecoatedELISAplate
• Fastandeasytoperform
• Lowbackground

AssayPrinciple:

Background:

ADAMTS-13

ADAMTS-13(adisintegrin-likeandmetalloproteinasewiththrombospondintype1motif13)isanenzyme(vWF-cleavingproteaseorvWF-CP)thatspecificallycleavesvonWillebrandFactor(vWF)multimers.