Description:
Technoview®UFHCalibratorKit includesplasma-derivedcalibration sampleswithunfractionatedheparin(UFH)concentrationsof ~0IU/mland~1.4IU/ml. Thecalibrators canbeusedfor establishingastandardcurveinresearchmeasurementsofheparin.Theyare optimizedforusewithanti-Xachromogenicassaysandare frequentlyusedinconjunctionwiththe Technoview®UFH lowandhighcontrolsinthe Technochrom®anti-Xachromogenicassay.
Advantages:
- Allowsforestablishmentofacalibrationcurvefor unfractionatedheparin
- Optimizedforusewithanti-Xa(withoutexogeneousantithrombin)research assays
CalibratorKitComposition:
2x1mlvialswithunfractionatedheparin (UFH) concentrations:
- Cal1:0IU/ml
- Cal5:1.4IU/ml
Calibratorsare lyophilizedandpreparedfrompooledhumancitratedplasmasupplementedwithdifferent concentrations(ornone)ofunfractionatedheparin.TheplasmacontainsstABIlizersbutnobactericideadditives.Theyare calibratedagainstthe5thInternationalStandard preparationforUFH97/578.
Background:
HeparinisawidelyusedantithromboticagentthatcanbeadmiNISTeredviaanintravenousorsubcutaneousroute.Itisnaturallyoccurringandtypicallyderived from bovineorporcinetissue. Theanticoagulanteffect ofthissulfatedglycosaminoglycanresultsfromitsbindingtoantithrombin(AT),themajorinhibitorofthecoagulationcascade.Bindinginducesaconformationalchange intheAT molecule,whichgreatlyacceleratestheAT inhibitionofseveralcoagulation proteases.TheacceleratedinhibitionofthrombinandFactorXabyheparinistheprimarybasisforitsanticoagulationproperties.
Twoformsofheparinareusedtherapeutically:unfractionatedheparin(UFH),withanaveragemolecularweight(MW)of15,000andlowmolecularweightheparin(LMWH),withaMWbetween4,000and6,500.ThelowerMWresultsinLMWHactingprimarilyonFactorXa,whereasUFHefficientlyinhibitsboththrombinandFactorXa. Bothheparinformsshouldbemonitored.UFHcanbemeasured withanaPTTorananti-XachromogenicassaywhileLMWHshouldbemeasuredusingandanti-Xachromogenicassay.NotethatthiscalibratorkitisforresearchuseonlyintheUSandCanada.
