Description:

Technoview®UFHCalibratorSet isasetofplasma-derivedcalibration sampleswithunfractionatedheparin(UFH)concentrations rangingfrom~0-1.4IU/ml. Thesetcanbeusedfor establishingastandardcurveinresearchmeasurementsofheparin. Itisoptimizedforusewithanti-Xachromogenicassaysand isfrequentlyusedinconjunctionwiththe Technoview®UFH lowandhighcontrolsinthe Technochrom®anti-Xachromogenicassay.

Advantages:

• Allowsforestablishmentofacalibrationcurvecoveringarangeof0-1.4IU/mlofunfractionatedheparin
• Optimizedforusewithanti-Xa(withoutexogeneousantithrombin)research assays

CalibratorSetComposition:

5x1mlvialswitharangeofunfractionatedheparin (UFH) concentrations:

• Cal1:0IU/ml
• Cal2:0.35IU/ml
• Cal3:0.70IU/ml
• Cal4:1.05IU/ml
• Cal5:1.40IU/ml

Calibratorsare lyophilizedandpreparedfrompooledhumancitratedplasmasupplementedwithdifferent concentrationsofunfractionatedheparin.TheplasmacontainsstABIlizersbutnobactericideadditives.Itiscalibratedagainstthe5thInternationalStandard preparationforUFH97/578.

Background:

HeparinisawidelyusedantithromboticagentthatcanbeadmiNISTeredviaanintravenousorsubcutaneousroute.Itisnaturallyoccurringandtypicallyderived from bovineorporcinetissue. Theanticoagulanteffect ofthissulfatedglycosaminoglycanresultsfromitsbindingtoantithrombin(AT),themajorinhibitorofthecoagulationcascade.Bindinginducesaconformationalchange intheAT molecule,whichgreatlyacceleratestheAT inhibitionofseveralcoagulation proteases.TheacceleratedinhibitionofthrombinandFactorXabyheparinistheprimarybasisforitsanticoagulationproperties.

Twoformsofheparinareusedtherapeutically:unfractionatedheparin(UFH),withanaveragemolecularweight(MW)of15,000andlowmolecularweightheparin(LMWH),withaMWbetween4,000and6,500.ThelowerMWresultsinLMWHactingprimarilyonFactorXa,whereasUFHefficientlyinhibitsboththrombinandFactorXa. Bothheparinformsshouldbemonitored.UFHcanbemeasured withanaPTTorananti-XachromogenicassaywhileLMWHshouldbemeasuredusingandanti-Xachromogenicassay.NotethatthiscalibratorsetisforresearchuseonlyintheUSandCanada.