Description:
Technoview®LMW calibratorkit includesplasma-derivedcalibration sampleswithlowmolecularweight heparin(LMWH)concentrationsof ~0IU/mland~1.6IU/ml. Thecalibrators canbeusedfor establishingastandardcurveinresearchmeasurementsofLMWheparin.Theyare optimizedforusewithanti-Xachromogenicassaysandare frequentlyusedinconjunctionwiththe Technoview®LMWheparinlow,mediumandhighcontrolsinthe Technochrom®anti-Xachromogenicassay.
Advantages:
- Allowsforestablishmentofacalibrationcurveforlowmolecularweight heparin
- Optimizedforusewithanti-Xa(withoutexogeneousantithrombin)research assays
CalibratorKitComposition:
2x1mlvialswithlowmolecularweight(LMW)heparinconcentrations:
- Cal1:0IU/ml
- Cal5:1.6IU/ml
Calibratorsare lyophilizedandpreparedfrompooledhumancitratedplasmasupplementedwithdifferent concentrations(ornone)oflowmolecularweight heparin.TheplasmacontainsstABIlizersbutnobactericideadditives.Theyare calibratedagainsttheInternationalStandard preparationforLMWH, 01/608.
Background:
HeparinisawidelyusedantithromboticagentthatcanbeadmiNISTeredviaanintravenousorsubcutaneousroute.Itisnaturallyoccurringandtypicallyderived from bovineorporcinetissue. Theanticoagulanteffect ofthissulfatedglycosaminoglycanresultsfromitsbindingtoantithrombin(AT),themajorinhibitorofthecoagulationcascade.Bindinginducesaconformationalchange intheAT molecule,whichgreatlyacceleratestheAT inhibitionofseveralcoagulation proteases.TheacceleratedinhibitionofthrombinandFactorXabyheparinistheprimarybasisforitsanticoagulationproperties.
Twoformsofheparinareusedtherapeutically:unfractionatedheparin(UFH),withanaveragemolecularweight(MW)of15,000andlowmolecularweightheparin(LMWH),withaMWbetween4,000and6,500.ThelowerMWresultsinLMWHactingprimarilyonFactorXa,whereasUFHefficientlyinhibitsboththrombinandFactorXa. Bothheparinformsshouldbemonitored.UFHcanbemeasured withanaPTTorananti-XachromogenicassaywhileLMWHshouldbemeasuredusingandanti-Xachromogenicassay.NotethatthiscalibratorkitisforresearchuseonlyintheUSandCanada.
