Description:
Technoview®LMWCalibratorSet isasetofplasma-derivedcalibration sampleswithlowmolecularweight heparin(LMWH)concentrations rangingfrom~0-1.6IU/ml. Thesetcanbeusedfor establishingastandardcurveinresearchmeasurementsoflowmolecularweightheparin. Itisoptimizedforusewithanti-Xachromogenicassaysand isfrequentlyusedinconjunctionwiththe Technoview®LMWheparin low,mediumandhighcontrolsinthe Technochrom®anti-Xachromogenicassay.
Advantages:
- Allowsforestablishmentofacalibrationcurvecoveringarangeof0-1.6IU/mloflowmolecularweight heparin
- Optimizedforusewithanti-Xa(withoutexogeneousantithrombin)research assays
CalibratorSetComposition:
5x1mlvialswitharangeoflowmolecular heparin(LMWH) concentrations:
- Cal1:0IU/ml
- Cal2:0.4IU/ml
- Cal3:0.8IU/ml
- Cal4:1.2IU/ml
- Cal5:1.6IU/ml
Calibratorsare lyophilizedandpreparedfrompooledhumancitratedplasmasupplementedwithdifferent concentrationsoflowmolecularweight heparin.TheplasmacontainsstABIlizersbutnobactericideadditives.Theyare calibratedagainsttheInternationalStandard preparationforLMWH,01/608.
Background:
HeparinisawidelyusedantithromboticagentthatcanbeadmiNISTeredviaanintravenousorsubcutaneousroute.Itisnaturallyoccurringandtypicallyderived from bovineorporcinetissue. Theanticoagulanteffect ofthissulfatedglycosaminoglycanresultsfromitsbindingtoantithrombin(AT),themajorinhibitorofthecoagulationcascade.Bindinginducesaconformationalchange intheAT molecule,whichgreatlyacceleratestheAT inhibitionofseveralcoagulation proteases.TheacceleratedinhibitionofthrombinandFactorXabyheparinistheprimarybasisforitsanticoagulationproperties.
Twoformsofheparinareusedtherapeutically:unfractionatedheparin(UFH),withanaveragemolecularweight(MW)of15,000andlowmolecularweightheparin(LMWH),withaMWbetween4,000and6,500.ThelowerMWresultsinLMWHactingprimarilyonFactorXa,whereasUFHefficientlyinhibitsboththrombinandFactorXa. Bothheparinformsshouldbemonitored.UFHcanbemeasured withanaPTTorananti-XachromogenicassaywhileLMWHshouldbemeasuredusingandanti-Xachromogenicassay.NotethatthiscalibratorsetisforresearchuseonlyintheUSandCanada.
