Description:
Technoclot®DTI isaresearchclottingassaykitforquantitativedeterminationofdirectthrombininhibitors(DTI)likedABIgatran and argatrobaninhumancitratedplasma. Technoclot®DTIisbasedonamodifiedThrombinTimedetermination.Thedilutedsampleismixedwithnormalplasmaandclottingisinitiatedbytheadditionofadefinedamountofthrombinreagent.Themeasuredclottingtimesareproportionaltoinhibitorconcentrationsandcanbedirectlyreadoffacalibratorspecificcalibrationcurves.
FormeasuringDTIsinplasma,inafirststepthedilutedsampleismixedwithnormalhumanplasmareagentR1.ClottingistheninitiatedbyaddingthethrombinreagentR2.TheclottingtimemeasuredisdirectlyrelatedtotheconcentrationoftheassayedDTIinthesampleplasma.
DabigatranistheactivesubstanceinPradaxa®whichisaregisteredtrademarkofBoehringerIngelheim.
KitComposition:
- 4x1mlReagent1(R1):NormalPlasma,lyophilized
- 2x2mlReagent2(R2):BovineThrombin26IU,stabilizer,polybrene,lyophilized.
MATERIALREQUIRED(notsuppliedwiththekit)
- Pipette:200μl
- Distilledwater
- Diluent,NaClsolution0.9%
- Calibrationplasma
- Catalog# 5090210Technoview®DabigatranCalibrators1-5,5x1ml
- Controlplasma
- Catalog# 5090212Technoview®DabigatranControlHigh,5x1ml
- Catalog# 5090214Technoview®DabigatranControlLow,5x1ml
MeasurementPrinciple:
Technoclot®DTIisbasedonamodifiedThrombinTimedetermination.Thedilutedsampleismixedwithnormalplasmaandclottingisinitiatedbytheadditionofadefinedamountofthrombinreagent.Themeasuredclottingtimesareproportionaltoinhibitorconcentrationsandcanbedirectlyreadoffacalibratorspecificcalibrationcurves.
