Description:

ChromogenixS-2366™isachromogenicsubstrateforfactorXIandactivatedproteinC.ThesubstratecanbeusedforthedeterminationofpurifiedenzymepreparationsaswellasofProteinCandFXIinplasma.

Chemicalname:L-Pyroglutamyl-L-prolyl-L-argininep-Nitroanilinehydrochloride.

Formula:<Glu-Pro-Arg-pNA·HCl

Mol.wt:539.0

e316nm:1.27·104mol-1·L·cm-1

Solubility:>10mmol/LinH2O

MeasurementPrinciple:

Themethodforthedeterminationofactivityisbasedonthedifferenceinabsorbance(opticaldensity)betweenthepNAformedandtheoriginalsubstrate.TherateofpNAformation,i.e.theincreaseinabsorbancepersecondat405nm,isproportionaltotheenzymaticactivityandisconvenientlydeterminedwithaphotometer.

Selectivity:

S-2366™isalsoreADIlysplitbytrypsin,thrombin,plasminandtissueplasminogenactivator.ItissplitbyFXIIa,plasmakallikreinandFXaaswell.Themethodforthedeterminationofactivityisbasedonthedifferenceinabsorbance(opticaldensity)betweenthepNAformedandtheoriginalsubstrate.TherateofpNAformation,i.e.theincreaseinabsorbancepersecondat405nm,isproportionaltotheenzymaticactivityandisconvenientlydeterminedwithaphotometer.