Description:
ChromogenixS-2302™isachromogenicsubstrateforplasmakallikrein.Thesubstratehasbeenusedforthedeterminationof:
- Prekallikreininplasma
- Kallikreininhibitorsinplasma
- FXIIinplasma
- Kallikrein-likeactivityinplasma
- Prekallikreinactivatorinalbuminandimmunoglobulinpreparations
Composition:
EachvialcontainschromogenicsubstrateS-2302™25mgandmannitol60mgasabulkingagent.StABIlity:Substance:Stableuntilexpirydateifstoredat2-8°C.Avoidexposuretolight.Thesubstanceishygroscopicandshouldbestoreddry.Solution:4mmol/linH2Oisstableformorethan6monthsat2-8°C.
Chemicalname:H-D-Prolyl-L-phenylalanyl-Larginine-p-nitroanilinedihydrochloride.
Formula:H-D-Pro-Phe-Arg-pNA·2HCl
Mol.wt:611.6
Selectivity:
S-2366™isalsoreADIlysplitbytrypsin,thrombin,plasminandtissueplasminogenactivator.ItissplitbyFXIIa,plasmakallikreinandFXaaswell.Themethodforthedeterminationofactivityisbasedonthedifferenceinabsorbance(opticaldensity)betweenthepNAformedandtheoriginalsubstrate.TherateofpNAformation,i.e.theincreaseinabsorbancepersecondat405nm,isproportionaltotheenzymaticactivityandisconvenientlydeterminedwithaphotometer.
