Description:
Ecarin50-B isforthedeterminationofprothrombinlevels.Basedontheinteractionofhirudinwithmeizothrombinasimpleecarinclottingtestforthemonitoringofhirudinlevelshasbeendeveloped.Duetoitsactiononacaroboxyprothrombin,ecarincanbeusedforthedeterminationofacarboxyprothrombininthesupernatantofbariumsulfatetreatedpatientplasmawherenormalcarboxylatedprothrombinhasbeenremovedbyadsorption.Ecarincanbeusedforthedetectionoflowprothrombinconcentrationsinplasmafractionsforqualityandin-processcontrolpurposes.Ecarin,usedinconjunctionwiththephospholipid-andcalciumion-dependantprothrombinactivatortextarinfromPseudonajatextillisvenom,formsahighlysensitiveandspecifictestsystemforlupusanticoagulant.
Origin
Echiscarinatussnakevenom
MW
55’000to60’000
Background:
Ecarinisametalloproteaseisolatedfromthevenomofthesaw-scaledviper(Echis carinatus)thatactivatesprothrombin.Ecarindoesnotaffectotherclottingfactors.Theaction ofecarinonprothrombinisindependentofcalcium,phospholipidsandfactorV.
