Description:

TheTechnozym®VitronectinAntigenELISAkitisanantibody“sandwich”systeminwhichamonoclonalactsasthecatchingantibodyandaperoxidase-labeledpolyclonalisthedetectingantibody.

KitComposition:

1. PLATE+PLATECOVER12×8wellmicrotitrestripsprecoatedwithamonoclonalanti-VNantibodyandblockedwith1%bovineserumalbumin(BSA),lyophilized.
   (TC-CodeKB)
2. STANDARD1xlyophilizednormalpooledhumanplasma.
   (TC-CodeDR)
3. POX-ANTIBODY
   1xconjugatedpolyclonalantiVNantibodies(concentrated).
   (TC-CodeDQ)
4. DILUTIONBUFFER–(whitecap)1x20ml2.5xconcentrated(PBS,1%BSA)
   (TC-CodeDS)
5. POXDILUTIONBUFFER–(whitecap)1x12mLPBS,1%BSA(readytouse)
   (TC-CodeDD)
6. SUBSTRATE–(greencap)1x12mlTMBSubstrate
   (TC-CodeKN)
7. STOPSOLUTION–(redcap)1x15mlSulphuricacid,0.45mol/l
   (TC-CodeKK)
8. WASHBUFFER–(bluecap)1x20ml12.5xconcentrated(PBS,0.5%Tween20)
   (TC-CodeBE)

Kitstorage:Storeallcomponentsat2-8°C.

MeasurementPriniciple:

TheTechnozym®testsystemforthedeterminationofvitronectinantigenisasolidphaseenzymeimmunoassay.

Background:

Vitronectin(Vn)isaglycosaminoglycanadhesiveglycoproteinandinitsintactformhasanapparentmolecularweightof78,000.BloodplasmaVnissynthesizedintheliverandispresentinaconcentrationrangebetween250µg/mland450µg/ml.VnistheprimaryPAI-1bindingproteinintheECMandplasma.PAI-1complexedwithVninplasmaandECMisfoundtobefullyactiveincontrasttoPAI-1insolutionwhereitisunstableandispresentinaninactive,latentform.ThusindicatingthattheinhibitorisstABIlizedthroughitsassociationwithcomponentsoftheECM,i.e.Vn.PAI-1bindstotheNH₂regionofVninthe“somatomedinB”domain.

EvidenceshowsVnasamodulatorofenzymespecificityofvariousserpinsthroughtheformationofternaryassociationproductsaswellasanovelproteincofactorforserpinfunctionthroughitsbinarycomplexformationwithPAI-1,wherebyitcandirectlyregulatetheenzymespecificityofPAI-1.