Description:

The Technochrom®C1-INHkitisachromogenicC1EsteraseInhibitorAssay.

Forresearchusein hereditaryangioedema(HAE).TheC1-esteraseinhibitor(C1-INH)isaregulatoryproteinthatfunctionsasaninhibitorofseveralserineproteasesinthecomplementsystem,thekallikrein-kininsystem,thecoagulationcascadeandinfibrinolysis.

AssayC1INHactivityinconcentrates

• UsedforcalibrationoftheInternationalStandardforpurifiedconcentratestandard(forbatchreleasetestingoftherapeuticproducts)

AssayC1INHactivityinplasma

• UsescoagulationreferenceplasmacalibratedagainstISforplasma

Forfullyautomated,bar-codedreagents,pleaseseetheCeveron® C1-INHAssay(5345005)foruseontheCeveron®alphaanalyzer.

Advantages:

• Assaylinearity20-120%
• Simpletestperformance
• Heparininsensitive
• Excellentaccuracyandprecision
• Adaptationsheetsforseveralanalyzersareavailable

MeasurementPrinciple:

C1-inhistitratedagainstanexcessofC1-esterasetoformaninhibitorycomplex,andtheresidualC1-esteraseactivityismeasuredusingachromogenicsubstrate.

C1-Esterase+C1-INH⎯⎯→[C1-Esterase.C1-INH]+C1-Esterase
((rest)
(excess)
C2H5CO-Lys(∈-Cbo)-(C1-Esterase)C2H5CO-Lys(∈-Cbo)-
Gly-Arg-pNA+H2O⎯⎯⎯⎯→Gly-Arg-OH+pNA”

Background:

TheC1esteraseinhibitorproteinisanormalconstituentofserumwhichfunctionsasaserineproteinaseinhibitoroftheserpinfamily.

TheC1esteraseinhibitorinhibitsthecomplementproteasesC1randC1s,aswellastheproteasesKallikrein,factorXIa,XIIaandplasminofthebloodclottingsystem.