Description:

RoxProthrombinisachromogenicassaykitforthequantitativedeterminationofProthrombin(FII)functionalactivityinplasmaandFIIcontainingconcentrates.ThemethodissuitableforplasmacollectedincitrateorEDTA.

• AchromogenicProthrombin methodbasedupontheprothrombinasecomplex.
• non-γ-carboxylated-FIIisnotactivatedinthemethodincontrasttosnakevenombasedprothrombinmethods.
• Detection Limit=about0.05U/mL(5%)whenusingaplasmadilutionof1:200as  prescribedinthepackageinsert.Thedetectionlimitforundilutedsamplesisabout0.25mU/mL.

KitComposition:

ActivatorReagent,3.0ml(4vials)(catalog#2010)
TheActivatorReagentcontainslyophilizedhumanFXa,bovineFVa,CaCl2andphospholipids.Eachvialissufficientfor25tests.

FIIaSubstrate,6ml(1vial)(catalog#2080)
LiquidsolutionofachromogenicFIIasubstrate(H-D-Phe-Pip-Arg-pNA),2.0mmol/L.

FIIDiluentBuffer,StockSolution,20ml(1vial)(catalog#2050)
Liquidstocksolutionofdiluentbuffer.

MeasurementPrinciple:

FIIfunctionalactivityisdeterminedinachromogenicprothrombinasemethod,inwhichhumanFIIisactivatedtothrombin(FIIa)byhumanFXainthepresenceofbovineFV,calciumionsandphospholipid.

TheamountofFIIaformedisdeterminedfromthehydrolysisofachromogenicFIIasubstrate.TheFIIactivityofthesampleisassignedvs.plasmaoraFIIconcentratestandardwithFIIpotencyexpressedinInternationalUnits(IU).

Theprothrombinasecomplexissensitivetog-carboxylationandthereforenon-γ-carboxylated-FIIisnotactivatedinthismethodincontrasttosnakevenombasedprothrombinmethods.

Background:

FactorIIisasinglechainvitaminKdependentglycoproteinof72kDa,whichisactivatedtothrombin(FIIa)byFXainthepresenceofFVa,calciumionsandphospholipids.