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Diapharma/Technoclone Anti-Protein C INH Antibody/TC21353/Vial/500µg
Description:
TechnocloneAnti-ProteinCINHAntibodyisamonoclonalantibody(mAB)4PCIthat bindstointactPCIandPCItargetenzymecomplexes.
Specificity
BindstointactPCIandPCI-targentenzymecomplexes.
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedurinaryPCIaccordingtothemethodof Geigeretal.(1).
Antibodyclass
IgG
Purification
Theantibodyispurifiedfrommurineascitesbyaffinity chromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Application
- Purificationfromurine[Eckeetal.(2)]
- ImmunodepletionofPCIfromhumanplasma
- ELISAtests
Composition:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand 20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused (e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower. Avoidrepeatedfreeze-thawcycles.
Literature:
- GeigerM.,PriglingerU.,GriffinJH.,B.R.BinderUrinaryProteinCInhibitor. GlycosaminoglycanssynthesizedbytheepithelialkidneycelllineTCI-598enhance itsinteractionwithurokinase.JBiolChem1991,266:11851-11857.
- EckeS.,GeigerM.ReschI.,JerabekI.,StinglL.,MaierM.andB.R.Binder. InhibitonofTissueKallikreinbyProteinCInhibitor.JBiolChem1992,267:inpress
