Description:

Technozym®tPA-PAI-1ComplexELISAisaresearchassay kitfordeterminationoft-PA-PAI-1complexesinplasma.Thet-PA-PAI-1Complexkitisbasedonamonoclonalantibodydirectedagainstt-PAasacatchingantibodyfort-PA-PAI-1complexesandaperoxidase-labeledmonoclonalanti-PAI-1antibodyusedforthedetectionofboundt-PA-PAI-1complexes. TheTechnozym®t-PA-PAI-1testisasolidphaseenzymeimmunoassay.

KitComposition:

•  PLATE+PLATECOVER:12×8wellmicrotitrestripsprecoatedwithamonoclonalantit-PAcoatingantibodyinbicarbonatebufferandblockedwith1%bovineserumalbumin(BSA)(TC-CodeGN).
• STANDARD:1xlyophilizedt-PA-PAI-1complexes(TC-CodeBT)
• POX-ANTIBODY:1xconjugatedmonoclonalanti-PAI-1antibody(concentrated)(TC-CodeKL)
• DILUTIONBUFFER: (whitecap)1x20ml2.5xconcentrated(PBS,1%BSA,5mMEDTA(TC-CodeAD).
• POXDILUTIONBUFFER: (whitecap)1x12mlPBS,1%BSA(TC-CodeDD).
• SUBSTRATE: (greencap)1x12mLTMB(Tetramethylbenzidine)insubstratebuffercontainingH2O2.Readytouse.(TC-CodeKN)
• STOPSOLUTION:1x15mlsulfuricacid0,45mol/l(TC-CodeKK)
• WASHBUFFER: (bluecap)1x20ml12×5xconcentrated(PBS0.5%,Tween20)(TC-CodeBE)

Background:

PlasminogenActivatorInhibitorTypeI(PAI-1)isthemajorinhibitoroftissuetypeplasminogenactivator(t-PA).Intherestingstate,thebody’splasmaconcentrationofPAI-1greatlyexceedsthet-PAplasmalevelandevenfollowingphysicalexertionorvenousocclusion,conditionswhichleadtoincreasedt-PAlevels,PAI-1isusuallyavailableinadequateamountstocompensateforincreasedactivatorlevels.Thus,increasesint-PA-PAI-1complexesaslongasactive,non-complexedPAI-1isavailable.Certainpathologicalconditionsareassociatedwithincreasedt-PAvalues.Theliveristhemajorsiteoft-PAdegradationandhepaticdisordersareoftenaccompaniedbyincreasedt-PAlevelsand,thus,increasedt-PA-PAI-1complexvalues.

Inhealthypersons,thet-PAlevelincreaseswithageandcomplexlevelsriseaccordingly.Thequantitationoft-PA-PAI-1complexesaidsintheinterpretationofalterationsinthelevelsoft-PAand/orPAI-1.Sincetheliver,inadditiontobeingasiteoft-PAdegradation,isanorganofPAI-1synthesisthequantitationofactivator-inhibitorcomplexesmayserveasandindicatoroftheextenttowhichactivePAI-1isavailabletocompensateforareductioninthehepaticdegradationoft-PA,i.e.elevationoft-PAplasmavalues.

Thedetectionofelevatedt-PA-PAI-1complexvaluesmaybeusefulfortheresearch ofliverdysfunctionswhichmayresultinclottingdisorders.