Description:
TechnocloneAnti-tPAPolyclonalAntibody,5mg
Reactionwithfreet-PAandt-PAinhibitorcomplexes,noreactionwithotherplasmaproteins.ApplicationsincludeimmunoblottingandELISAtests.
Immunizingantigen
PurifiedHumanGlu-Plasminogen
Immunizedspecies
Rabbit
Purification
TheantibodyispurifiedfromrabbitserumbyammoniumsulphatepurificationfollowedbyDEAEion-exchangechromatographyandpurityistestedbySDS-PAGE.
Characteristicsoftheantibody
ReactswithPlasminogenanditscomplexes.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof1mgandshouldbereconstitutedwith1mldistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreez-thawcycles.