Description:
TechnocloneAnti-tPAMonoclonalAntibody7VPA
IgG1;halfmaximalbindingtocoatedt-PAat19µg/ml(lowaffinity);Reactionwithanepitopeexpressedonkringle2.Competitiveinhibitionofplasminogenactivationinthepresenceoffibrin(Ki=0.57nM). Itcanbeusedforbiochemicalstudies.
SPECIFICITY
Bindstot-PA.ReactionwithanepitopeexpressedonKringle2.Competitiveinhibitionofplasminogenactivationinthepresenceoffibrin(Ki=0.57nM).Halfmaximalbindingtocoatedt-PAat19.0µg/ml(lowaffinity).
DERIVATION
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedmelanoma-typet-PA.
ANTIBODYCLASS
IgG1
PURIFICATION
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
