Description:
TechnocloneAnti-scu-PAMonoclonalAntibody35scu-PA
IgG1;bindstosinglechainurokinase,two-chainurokinase,andlowmolecularweighturokinase.Noeffectonthefunctionalactivityoftwo-chainurokinase. Applicationsinclude ELISAtestsandimmunohistochemistry(1)
Presentation
Lyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline(PBS)pH7.4IsotypeIgG1,κlightchain.
Specificity
35scu-PAbindsto:
- single-chainpro-urokinase(scuPA)
- two-chainurokinase-typeplasminogenactivator(uPA)
- lowmolecularweighturokinase
Purification
AffinitychromatographyonProteinGsepharoseImmunogenRecombinantsingle-chainpro-urokinase(scuPA)GenerationofhybridomacellsNSOmyelomacelllineXspleencellsBALB/cmice
Suggestedworkingconcentration
10µg/ml.
Handlingandstorage:
Reconstitutewith0,5mLdistilledwaterforpreparinghighdilutionsuseaproteincontainingdiluent(e.g.1%BSAinPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
Literature:
1)Plas,E.,Carroll,V.A.,Jilch,R.,Mihaly,J.,Vesely,M.,Ulrich,W.,Pfluger,H.,andBinder,B.R.1998.AnalysisoffibrinolyticproteinsinrelationtoDNAploidyinprostatecancer.Int.J.Cancer78:320-325
