Description:
TechnocloneAnti-alpha2-Antiplasmin14AP
Lyophilized.Monoclonal.IgG2a;inhibitsalpha-2-antiplasminactivity inafunctionalassaysystem.
Specificity
Recognizesfunctionalα-2-antiplasmin.
Derivation
HybridiationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedα-2-antiplasmin.
Antibodyclass
IgG2a
Purification
TheantibodyispurifiedfromhybridomasupernatantsbyaffinitychromatographyonimmobilizedrecombinantProteinG.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
α-2-antiplasminInhibition:
- 14APat100µg/mlpreincubatedfor15minuteswithα-2-antiplasminat37°C
- Plasminaddition.Incubatedfor15minutesat37°C
- Measureduncomplexedplasminwithaplasminsubstrate(S-2251)for15minat37°C
- Recordedopticaldensityat405nm(OD405)
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mLdistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
