Description:
TechnocloneAnti-PlasminogenMonoclonalAntibody4PG
IgG1;positivereactionwithmini-plasminogen,noreactionwithkringles1-3orkringle4asdemonstratedbyimmunoblotting;reactionwithfree,butnotcomplexed,plasminogen-plasmin. ApplicationsincludebiochemicalandpharmacologicalstudiesandantibodyforanELISAspecificforplasminogen.
Specificity
Positivereactionwithminiplasminogen,noreactionwithkringles1-3orkringle4asdemonstratedbyimmunoblotting;reactionwithplasminogenandfreeplasmin,butnotwithplasmin/antiplasmincomplexes(1).
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedhumanplasminogen.
Antibodyclass
IgG1
Purification
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
Literature:
1)E.Hattey,J.Wojta,andB.R.Binder:Monoclonalantibodiesagainstfourdifferentepitopesofplasminogenexhibitdifferenteffectsonplasminogenactivationkinetics.ThrombosisandHaemostasis58,No.1:440,Abstr.1622,1987
