Description:
TechnocloneAnti-PlasminogenMonoclonalAntibody7PG
IgG1;positivereactionwithfreeplasminogenorplasminincomplexwithAlpha-2-Antiplasmin.Directedagainstanepitopeonthekringle4elastasefragmentofplasminogen. Applicationsincludebiochemichalandpharmalogicalstudies.
Specificity
PositivereactionwithfreePlasminogenorPlasminincomplexwithα-2-antiplasmincomplexes.Directedagainstanepitopeonthekringle4elastasefragmentofplasminogen(1).
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedhumanplasminogen.
Antibodyclass
IgG1
Purification
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mLdistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
Literature:
1)E.Hattey,J.Wojta,andB.R.Binder:Monoclonalantibodiesagainstplasminogenandα-2-antiplasmin:Bindingtonativeandmodifiedantigens.ThrombosisResearch45:485,1987
