Description:
TechnocloneAnti-PlasminogenMonoclonalAntibody2PG
IgG1;positivereactionwithGlu-andLys-PlasminogenaswellaswithPlasmin-Alpha-2-Antiplasmincomplexes.Directedagainstanepitopeonthekringle1-3elastasefragmentofplasminogen.HalfmaximalbindingtocoatedGlu-Plasminogenat19ng/ml.
Applicationsincludesecondantibodyfordeterminationofplasmin-α-2-antiplasmincomplexinanELISAandbiochemichalandpharmalogicalstudies.
Specificity
PositivereactionwithGlu-andLys-PlasminogenaswellaswithPlasmin-α-2-antiplasmincomplexes.Directedagainstanepitopeonthekringle1-3elastasefragmentofplasminogen(1).HalfmaximalbindingtocoatedGlu-Plasminogenat10ng/ml.
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedhumanplasminogen.
Antibodyclass
IgG1
Purification
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
Literature:
1)E.Hattey,J.Wojta,andB.R.Binder:Monoclonalantibodiesagainstplasminogenandα-2-antiplasmin:Bindingtonativeandmodifiedantigens.ThrombosisResearch45:485,1987
