Description:
TechnocloneAnti-PAI-1PolyclonalAntibody,1mg
ReactionwithPAI-1fromendothelialcells,plateletsandhumanplasmaaswellaswithPAI-1fromconditionedmediaofthehumancelllinesMJZJandHT1080,recognizesfreeandcomplexedPAI-1aswellaslatentPAI-1. CanbeusedasanantibodyinanELISAforthedeterminationofPAIantigenandforimmunoblottingexperiments.
Immunizingantigen
HumanrecombinantPAI-1.
Immunizedspecies
Rabbit
Purification
TheantibodyispurifiedfromrabbitserumbyammoniumsulphateprecipitationfollowedbyDEAEion-exchangechromatography.
Characteristicsoftheantibody
ReactswithPAIfromendothelialcells,plateletsandhumanplasmaaswellaswithPAIfromconditionedmediaofthehumancelllinesMJZJandHT1080;recognizesfreeandcomplexedPAIaswellaslatentPAI.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mLsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mLmannitol.Itissuppliedinvialsof1mgandshouldbereconstitutedwith1mLdistilledwater.Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
