Description:

TechnocloneAnti-PAI-1PolyclonalAntibody,5mg

ReactionwithPAI-1fromendothelialcells,plateletsandhumanplasmaaswellaswithPAI-1fromconditionedmediaofthehumancelllinesMJZJandHT1080,recognizesfreeandcomplexedPAI-1aswellaslatentPAI-1. CanbeusedasanantibodyinanELISAforthedeterminationofPAIantigenandforimmunoblottingexperiments.

Immunizingantigen

HumanrecombinantPAI-1.

Immunizedspecies

RabbitPurificationTheantibodyispurifiedfromrabbitserumbyammoniumsulphateprecipitationfollowedbyDEAEion-exchangechromatography.

Characteristicsoftheantibody

ReactswithPAIfromendothelialcells,plateletsandhumanplasmaaswellaswithPAIfromconditionedmediaofthehumancelllinesMJZJandHT1080;recognisesfreeandcomplexedPAIaswellaslatentPAI.

Handlingandstorage:

Theantibodyislyophilizedfroma5mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mLmannitol.Itissuppliedinvialsof1mgandshouldbereconstitutedwith5mldistilledwater.Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.