Description:
TechnocloneAnti-PAI-1MonoclonalAntibody5PAI
IgG1;reactionwithactiveandlatentPAI-1andt-PA-PAI-1complexes;nocross-reactionwithPAI-2orwithPAI-3;interfereswiththefunctionalactivityofPAI-1.ApplicationsincludeimmunodepletionofPAI-1fromhumanplasmaandimmunopurificationinELISAtestsandimmunohistochemistry.
SPECIFICITY
BindstoactivePAI-1,tolatentPAI-1,toinactivePAI-1andtot-PA-PAI-1complexes.NobindingtoPAI-2orPAI-3.
DERIVATION
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedPAI-1fromthehumanmelanomacelllineMJZJaccordingtothemethodofWagneretal.(1).
ANTIBODYCLASS
IgG1
PURIFICATION
TheantibodyispurifiedfrommurineascitesbyammoniumsulphateprecipitationfollowedbyaffinitychromatographyonproteinGsepharose.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumacide.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
Literature:
1)O.F.Wagner,M.Vetterlein,andB.R.Binder:PurificationofanActivePlasminogenActivatorInhibitorImmunologicallyRelatedtotheEndothelialTypePlasminogenActivatorInhibitorfromtheConditionedMediaofaHumanMelanomaCellLine.J.Biol.Chem.261:14474,1986.
