Description:
TechnocloneAnti-alpha2-Antiplasmin3AP
IgG1;reactionwithnativealpha-2-antiplasminandwiththeMW=8,000peptidederivedbySDSgelelectrophoresisofplasmin-alpha-2-antiplasmincomplexesasdemonstratedbyimmunoblotting.Halfmaximalbindingtocoatedalpha-2-antplasminat8ng/ml.Theantibodycanbeusedfordetectionofuncleavedα-2-antiplasminandseparationoftheplasminogenbindingformofα-2-antiplasminfromthenon-plasminogenbindingform.
Specificity
Bindsnativeα-2-antiplasminandtheMr=8000peptidederivedbySDS-gel-electrohporesisofplasmin-α-2-antiplasmincomplex(1).Halfmaximalbindingtocoatedα-2-antiplasminat8ng/ml.
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedα-2-antiplasmin(1).
Antibodyclass
IgG1
Purification
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mLdistilledwater.Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
Literature:
1)E.Hattey,J.Wojta,andB.R.Binder:Monoclonalantibodiesagainstplasminogenandα-2-antiplasmin:Bindingtonativeandmodifiedantigens.ThrombosisResearch45:485,1987
