Description:
TechnocloneAnti-scu-PAMonoclonalAntibody14scu-PA
IgG1;bindstosinglechainurokinase,two-chainurokinase,andlowmolecularweighturokinase.Partiallyinhibitsthefunctionalactivityoftwo-chainurokinaseatappropriateconcentrations. Applicationsincludeimmunoblotting,ELISAtestsandenzymeinhibition.
Specificity
Bindstosinglechainurokinase,two-chainurokinaseandlowmolecularweighturokinase.Inhibitsthefunctionalactivityoftwo-chainurokinaseatappropriateconcentrations.
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithrecombinanthumansinglechainurokinase.
Antibodyclass
IgG1,Kappalightchain
Purification
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Inhibitionofurokinase
- 14scu-PAatconcentrationsrangingfrom0-10µg/mlwaspreincubatedwith20IU/mlurokinasefor15minutesat37°C
- Urokinaseactivitywasdetectedwithachromogenicsubstrate(S-2444)
- Opticaldensitywasreadafter15minutesincubationwiththechromogenicsubstrateat37°C
| 14scu-PAconc.µg/ml | %inhibition |
| 2.5 | 17 |
| 5 | 13.6 |
| 10 | 10 |
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mLmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
