Description:
TechnocloneAnti-scu-PAMonoclonalAntibody1scu-PA
IgG1;recognizesingleandtwochainurokinaseasdeterminedinanELISA.Applicationsinclude ELISAtestsandimmunoblotting.
Specificity
1scu-PAbindstosinglechainurokinaseandtwo-chainurokinase.
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithrecombinanthumansinglechainurokinase.
Antibodyclass
IgG1
Purification
TheantibodyispurifiedbyammoniumsulphateprecipitationfollowedbyFPLCseparation.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsalinepH7.4.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
