Description:
TechnocloneAnti-VitronectinMonoclonalAntibody2VN
IgG;bindstohumanvitronectin. ApplicationsincludeELISAtestsandimmunoblotting.
Specificity
BindstohumanVitronectin.
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedhumanVitronectin.
Antibodyclass
IgG
Purification
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
