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Diapharma/Technoclone Anti-Plasminogen Monoclonal Antibody 1PG/TC21103/Vial/500µg
Description:
TechnocloneAnti-PlasminogenMonoclonalAntibody1PG
IgG1;positivereactionexclusivelywithGlu-plasminogen,inimmunoblottingexperimentsnoreactionwithLys-plasminogen;reactionwithPlasmin-Alpha-2-AntiplasmincomplexesonlywhenGlu-formsareinvolvedincomplexformation.HalfmaximalbindingtocoatedGlu-plasminogenat6ng/ml.
Applicationsincludebiochemicalandpharmacologicalstudies,differentiationbetweenGlu-andLys-formsofplasminogen,antibodyforanELISAspecificforGlu-Plasminogen.
Specificity
Directedagainstthepreactivationpeptide.PositivereactionwithGlu-PlasminogenandGlu-Plasmin.NoreactionwithLysPlasminogen.ReactionwithPlasmin-α-2-antiplasmincomplexesonlywhenGlu-formsareinvolvedincomplexformation(1).HalfmaximalbindingtocoatedGlu-Plasminogenat6ng/ml.
Derivation
HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedhumanplasminogen.
Antibodyclass
IgG1
Purification
TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.
Handlingandstorage:
Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mLdistilledwater.
Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).
Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.
Literature:
1)E.Hattey,J.Wojta,andB.R.Binder:Monoclonalantibodiesagainstplasminogenandα-2-antiplasmin:Bindingtonativeandmodifiedantigens.ThrombosisResearch45:485,1987
