Description:

TechnocloneAnti-tPAMonoclonalAntibody2VPA

IgM;halfmaximalbindingtocoatedt-PAat650ng/ml(highaffinity);reactionwithfreet-PAandt-PA-PAI-1complexes;nocross-reactionwithurokinase.Directedagainstanepitopeonthelightchainoft-PAawayfromtheactivesite.ItstimulatesplasminogenactivationinthepresenceofCNBrfragmentsoffibrinogenandofplasminogenactivationintheabsenceofCNBrfragmentsoffibrinogen. ItcanbeusedinanELISA-systemascatchingantibody.Recommendedcoatingconcentration10µg/ml.

Specificity

Bindstofreet-PAandt-PA-PAIcomplexes.Reactionwithanepitopeexpressedonthelightchainoft-PAdifferentfromtheactivesite.Nocrossreactionwithurokinase.StimulationofplasminogenactivationinthepresenceandintheabsenceofCNBrfragmentsoffibrinogen.Halfmaximalbindingtocoatedt-PAat650ng/ml(highaffinity).

Derivation

HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedmelanoma-typet-PA.

Antibodyclass

IgM

Purification

Theantibodyispurifiedfrommurineascitesbymultipleprecipitationswith1%boricacid.

Handlingandstorage:

Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mLdistilledwater.

Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).

Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.