Description:

TechnocloneAnti-uPAMonoclonalAntibody4UK

IgG₁;noreactionwitht-PA,positivereactionwithu-PAandsinglechainpro-urokinaseastestedbyELISA.Halfmaximalbindingtocoatedurokinaseat560ng/ml.Noinhibitionofu-PAplasminogenactivatingactivity.

Specificity

Bindstotwo-chainurokinaseandsinglechainprourokinase(1).

Derivation

HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithhumanurinaryhighmolecularweighturokinasepurifiedaccordingtothemethodofHuberetal.(2).

Antibodyclass

IgG₁

Purification

TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.

Handlingandstorage:

Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsalinepH7.4.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.

Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).

Lyophilizedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.

Literature:

1)J.Wojta,J.C.Kirchheimer,L.Turcu,G.ChristandB.R.Binder:Monoclonalantibodiesagainsthumanhighmolecularweighturinaryurokinase:Applicationforaffinitypurificationofurinaryprourokinase.ThrombosisandHaemostasis55(3):347,1986.

2)K.Huber,J.Kirchheimer,B.R.Binder:Rapidisolationofhighmolecularweighturokinasefromnativehumanurine.Thromb.Haemost.47:197,1982