Description:
TechnoclonePurifiedFibronectinisfibronectinpurifiedfromhumanplasma,lyophilized. Forusein:
Tissue
Coatingofcultureplatesbeforeseedingofendothelialcellsresultsinbettergrowthspatternofseededcellsascomparedtountreateddishes(4)Recommendedconcentration:10µg/ml
Biochemicalstudies
Fibronectinanditsfragmentscanbeusedforstudiesontheeffectsoffibronectininprotein-proteininteractions(5)andproteincellinteractions(bindingtointegrins(6),cellactivation(7)etc.)
Composition:
Preparedfromhumanplasmapools.Purificationbyaffinitychromatographyofplasmaongelatinesepharosefollowedbyargininsepharose.TechnoclonePurifiedFibronectinissuppliedin1mgquantitieslyophilizedfromabuffer0.05MTris,0.15MNaCl,0,03%NaN3,pH7.4.
Reconstitution:
Thelyophilizedproductisreconstitutedbyadditionof1mLdistilledwaterandgentleagitation10minutesatroomtemperature.
| 1 | Appearance | Lyophiliziedwhitepowder |
| 2 | ProteincontentOD280 | 1mg |
| 3 | Purity(1) | TypicaldoublechainproteinbandwithMr~440kDwithoutreductionandofMr~220kduponreduction |
| 4 | Specificantigen(2) | ~1mgReactivewithmonoclonalantibodiesagainstintactuncleavedFibronectin(2FN)aswellaswithmonoclonalantibody(6FN)recognizingspecificepitopesintheFNmolecule(3) |
