Description:
Technoclot®PTOwren (automated)isathromboplastinreagentmodifiedaccordingtoOwrenforthequantitativedeterminationofProthrombinTime(PT)inhumancitratedplasma.ThereagentisenrichedwithbovineplasmafromwhichtheprothrombincomplexfactorsII,VIIandXhavebeenremoved.ThebovineplasmaremainsasourceoffibrinogenandfactorV,whichthereforecannotbedetectedbythetest. ThisreagentissensitivetoabnormallevelsofthecoagulationfactorsII,VIIandXandisusedfortheresearch oforal anticoagulants(i.e.warfarin).Technoclot®PTOwrenautomatedisespeciallydesignedtobeusedwith coagulationanalyzersincludingonesusingopticalPTdetermination.
Technoclot®PTOwrenreplaces Thrombotest®andNormotest®.
Composition:
Thereagentislyophilizedandcontainsrabbitbrainthromboplastinandadsorbedbovineplasma.Theadsorbedplasma isaddedasasourceoffactorVandfibrinogen.
25mmCalciumchloridesolutionneedstobeaddedtoinitiatetheclottingreaction.
Materialrequired(notsuppliedwiththekit)
- Pipettes
- Solutions
- Distilledwater
Imidazolebuffer*25ml, Catalog# 5410008
Ca-Chloridesolution25mm*25ml, Catalog# 5277017 - ControlPlasma*
CoagulationControlN,5x1ml, Catalog# 5020040
CoagulationControlAK,5x1ml, Catalog# 5011050
CoagulationControlA,5x1ml, Catalog# 5021055 - CalibrationPlasma*
AK-Calibrant,4x1ml, Catalog# 5010004
CoagulationReference,5x1ml, Catalog# 5220110
*oranyotherpackagesizesorTechnoclot®reagents.