Description:

Technoclot®LA(LupusAnticoagulant)Screenis asimplified(one-stage)diluteRussell’sViperVenom(dRVV)test.Itiscomprisedofasimplified,completesystemforthedetectionofLupusAnticoagulant.

Composition:

Technoclot®LAScreenandTechnoclot®LAConfirmcontainRussell’svipervenom,phospholipidsantiheparinagents,calcium,buffers,stABIlizers,sodiumazideanddyes.

MeasurementPrinciple:

Russell’svipervenompresentinLAScreeninitiateplasmaclottingbydirectlyactivatingfactorX.LAantibodiesprolongtheLAScreenclottingtime.

Technoclot®LAConfirmissimilartoTechnoclot®LAScreenbutcontainsahighphospholipidconcentration.TheextraphospholipidcounteractstheLAantibodyandlargelycorrectstheclottime.

DRVVtests“bypass”factorVIIoftheextrinsicpathwayandthecontactandantihemophilicfactorsoftheintrinsicpathway.ThereforeTechnoclot®LAScreenismorespecificforLAthanaPTTsastheyarenotaffectedbycontactfactorabnormalitiesorbyfactorFVIIIdeficienciesorantibodies.TheyhavebeenanumberoftestsdevelopedbasedonphospholipidcorrectionhowevernonehavebeenasconvenienttouseasLAConfirmsubsequenttoLAScreen.

MixingtestsmaybeusefultoexcludefactorII,VandXdeficiencies,whichmayprolongLAScreenandLAConfirmresults.Mixingnormalplasmawithtestplasmareplenishesanyfactorsdeficientinthetestplasma.Ifthemixingtestisstillprolonged,itindicatesthataninhibitor(suchasLA)ispresentinthetestplasma.