Description:

TechnocloneLupusAnticoagulantTestisareagentkitfordeterminationofLupusinhibitor.ModifiedaPTTsareperformedinusingtwodifferentphospholipidconcentrationseachinaplasmaexchangeassayoftheplasmasamplewithnormalplasma.

LupusinhibitorsdirectedagainstplateletfactorIII(phospholipids)canbedetectedbymeansofamodifiedactivatedpartialthromboplastintime(aPTT).InthetestanactivatorofSiO₂/AI₂O₃sUSPensionandtwophospholipidconcentrationsareused.ThemodifiedaPTT’sareperformedwithnormalplasma(freefromplateletcontamination),withpatientplasmaandwithamixtureofbothplasmas.Fromtheshapeofthe“aPTTagainstplasmaconcentration”curvetheprobABIlityofthepresenceoflupusinhibitorcanbeassessed.

KitComposition:

• ReagentA:1x3,phospholipid
• ReagentB:2x3,SiO2/AI2O3-suspension
• ReagentC:2x2,plateletpoornormalplasma
• LupusInhibitorplasma low:1x1,positivecontrol

LupusInhibitorPlasmaLowispreparedfromselectedlupusinhibitorplasmapositiveinmostLupusInhibitorscreeningandconfirmatoryassays.LupusInhibitorPlasmacontainsstabilizersbutnobactericideadditives.

MATERIALREQUIRED
(notsuppliedwiththekit)

–Pipette
–Distilledwater
–Buffer(catalog# 5279025CaCl250mmol/lsolution100ml)