Description:
ChromogenixCoamatic®ProteinCisa chromogenicassaykitforthequantitativedeterminationofproteinCactivityinhumancitratedplasma.
ProteinCplaysanimportantroleintheanticoagulantregulatorymechanisms.Hereditaryand/oracquiredProteinCdeficiencyhasbeenshowntobeariskfactorfordevelopmentofvenousthrombosis.
Performance:
Noinfluenceofheparinlevelsupto3IU/ml.Simpleandrapidperformancegivesoptimaluserconvenience.ExcellentaccuracyandprecisionaswellasreagentstABIlity.Validatedanddocumentedprotocolsforawiderangeofinstruments.Suitableforbothlargeandsmalllaboratories.
KitComposition:
Reagents, Packaging,StorageandStability
- S-2366™6mg,2vials.LyophilizedchromogenicsubstratepyroGlu-Pro-Arg-pNA·HCl.Stabilityafterreconstitution:3monthsat2-8°Cintheoriginalvial.
- ProteinCactivator1.2U,2vials.LyophilizedvenomenzymefromSouthernCopperheadSnake(AgkistrodonContortrixContortrix),withbovineserumalbumin(stabilizer)andCiprofloxacin®(preservative).Stabilityafterreconstitution:3monthsat2-8°Cintheoriginalvial.
Thesealedreagentsarestableat2-8°Cuntiltheexpirydateprintedonthelabel.WARNING:Donotusereagentsbeyondtheexpirydateprintedonthepackagelabel.Discardifthesubstratesolutionappearsyellow.
Determinations:
- Testtube:72
- Microplate:288
- Automated:upto180
MeasurementPrinciple:
ProteinCinplasmaisactivatedbyaspecificenzymefromSouthernCopperheadSnakevenom.TheamountofactivatedproteinCisdeterminedbytherateofhydrolysisofthechromogenicsubstrateS-2366™.ThepNAreleasemeasuredat405nmisproportionaltotheProteinClevelintherangefrom0-120%ofnormalplasma.
| ProteinCactivator | ||
| ProteinC | ——→ | ActivatedProteinC(APC) |
| APC | ||
| S-2366™ | ——→ | Peptide+pNA |
Background:
ProteinCisavitaminKdependentplasmaproteinwhichplaysanimportantroleintheanticoagulantregulatorymechanisms.Itcirculatesasazymogenandisconvertedtoanactiveserineprotease,activatedProteinC(APC),bytheactionofthrombininpresenceofthrombomodulin.APCregulatesthecoagulationsystembyproteolyticcleavageandinactivationofactivatedfactorsVandVIII.Hereditaryand/oracquiredProteinCdeficiencyhasbeenshowntobeariskfactorfordevelopmentofvenousthrombosis.Readmore…
