Description:
ChromogenixCoamatic®LRAntithrombinisanassaykitforthequantitativedeterminationoftheheparincofactoractivityofantithrombin(AT)inhumancitratedplasma.Allcomponentsoftheantithrombintestkitareinliquidformulation(LR=LiquidReagents).
KitComposition:
Reagents,Packaging,StorageandStABIlity
- SubstratesolutionS-2772™,26mg,2vialsContainschromogenicsubstrateS-2772™(Ac-D-Arg-Gly-Arg-pNA·2HCl)intartaricacidbufferpH4.2.Stabilityonceopened:6monthsat2-8°Cintheoriginalvial.
- FactorXasolution,90nkat,6vialsContainspurifiedbovineFactorXa,bovinealbuminandheparin,inTrisbuffer,pH8.2.Stabilityonceopened:1monthat2-8°Cintheoriginalvial.
Unopenedreagentsandcalibratorarestableuntiltheexpirationdateshownonthevialwhenstoredat2-8°C.WARNING:Donotusereagentsbeyondtheexpirydateprintedonthepackagelabel.Discardifthesubstratesolutionappearsyellow.Avoidcontaminationbymicroorganisms.
MeasurementPrinciple:
| AT+Heparin | ————–> | [AT·Heparin] |
| [AT·Heparin]+FXa(excess) | ————–> | [AT·Heparin·FXa]+FXa(residual) |
| FXa(residual) | ||
| S-2772™ | ————–> | Peptide+pNA |
AT=Antithrombin
Background:
Antithrombinisthemostimportantnaturalinhibitorofthecoagulationcascade.Byinhibitingthecoagulationproteases,especiallythrombin,factorXaandfactorIXa,antithrombinpreventsuncontrolledcoagulationandthrombosis.PlasmaisincubatedwithanexcessofFactorXa(FXa)inthepresenceofheparin.TheresidualactivityofFXaisdeterminedbytherateofhydrolysisofthechromogenicsubstrateS-2772™.ThepNAreleasemeasuredat405nmisinverselyproportionaltotheATlevelintherange15-125%ofnormalplasma. Readmore…
