Description:
Technozym®PAI-1Actibind®ELISAisa highsensitivecompletesandwichELISAforthequantitativedeterminationofactivePAI-1antigeninhumanplasma.ThekitallowsdeterminationofactivePAI-1antigeninplasmaalsowithresearchdisseminatedintravascularcoagulationandinresearchofatherioscleroticdisease.ThePlasminogenActivatorInhibitortype1(PAI-1)ActibindELISAKitisbasedontheimmobilizationoffunctionallyactivet-PAtoplatesbymeansofamonoclonalantibody.PAI-1containedinthetestsamplesbindstot-PAandisthenquantifiedusingaperoxidase-labeledmonoclonalanti-PAI-1antibody.TheassayrecognizesPAI-1activeformwithnointerferencefromPAI-2(5U/mL)andPAI-3(5.5µg/mL).Acidifiedcitratedplasma,citrated,CTADorEDTAplasmascanbeused.TheassayisstandardizedwithInternationalstandard87/512.Assaytimeisonly60minutes.
Kitcontentsincludestandards,highandlowcontrols,coatedplate,peroxidase-labeledantibody,buffersandreadytouseTMBsubstrate.
KitComposition:
Reagents,Storageand StABIlity
- ELISAteststrips:(12)with8wellseach,precoatedwithtPAimmobilizedviaamonoclonal antitPAcoatingantibody;inanaluminiumbag.Stable afteropening untilexpirationdateifstored2-8°C withadhesivefilminplasticbagwithdryingagent.
- Washingbufferconcentrate:(PBS;pH7.3)containingdetergent;0.01%merthiolat;1bottle, 80ml.Washingbuffer(1+11.5dilutionofconcentrate)isstablefor 3weeksifstored 2-8°C.Washingbufferconcentrateisstablefor6monthsafteropening ifstored2-8°Cafter reconstitution.
- Incubationbuffer:(PBS;pH7.3);containsstabilizerprotein;0.05%proclin;anddye,1bottle, 90ml,readyforuse.Stableafteropeningfor2monthsifstored 2-8°C.
- Calibrators:(Standards)numbered;lyophilized;1bottleeach.Concentrationsarelotdependent; consultlabelonthevial.Stablefor6monthsifstored-20°Cafter reconstitution.
- Controlplasmas:“lowlevel”and“highlevel”forcheckingpurposeslyophilized;1bottleeach. Concentrationsarelot-dependent;consultthelabelonthevial. Stablefor6monthsifstored-20°Cafter reconstitution.
- Conjugate:monoclonalAnti-PAI-1-POX, dyedblue;1bottle,0.3ml. Stableafteropeningfor6 monthsifstored 2-8°C.Aworkingsolutionisstableatroomtemperaturefor60minutes.
- ChromogenTMB:(tetramethylbenzidine);1bottle,12ml;readyforuse.Stableuntilexpirationdateafteropeningifstored2-8°C.
- Stoppingsolution:sulphuricacid0.45mol/l;1bottle12ml;readyforuse.
- Adhesivefilm:forELISAteststrips(2).
Theexpirydateprintedonthelabelsappliestostorageoftheunopenedbottlesat+2-8°C.
