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当前位置: 首页 > 产品中心 > Other_kits > Diapharma/Chromogenix Coatest®APC™ 阻力–;VS/K823138/Kit/40-80测试
商品详细Diapharma/Chromogenix Coatest®APC™ 阻力–;VS/K823138/Kit/40-80测试
Diapharma/Chromogenix Coatest®APC™ 阻力–;VS/K823138/Kit/40-80测试
Diapharma/Chromogenix Coatest®APC™ 阻力–;VS/K823138/Kit/40-80测试
商品编号: K823138
品牌: Diapharma
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description:

ChromogenixCoatest®APC™Resistance–VSisanAPTT-basedassaykitforthescreeningoffactor-V-relatedAPCresistance.ThehighsensitivityandspecificityofthetestforthefactorV:Q506mutationisobtainedbypredilutingthesampleplasmawithanexcessofV-DEFPlasmabioreagent.ThetestdesignmakesitpossIBLetodiscriminatebetweenheterozygousandhomozygousfactorVgenotypes.Italsoallowsforanalysisofplasmafrompatientsonheparinororalanticoagulanttherapy.Highdiscriminationbetweengenotypeswith100%sensitivityforFV:Q506.ReducesneedforPCRdetermination.Applicabletoanticoagulanttreatedpatients.

Atime-andmoney-savingalternativetoindividualgenetictesting,Coatest©APC™Resistancetestingisanidealsolutionforphysiciansandlabs.Eachkitwasdesignedtobeeasytouseforin-housescreening,offeringfastresultsandlowcostsforbothlarge-andsmall-volumelabs.ThemostcommonFVLeidenscreeningtestperformed,itoffersunmatchedsensitivityfortheFV:Q506mutation–closeto100%specificity–andisapplicabletopatientsonheparinorwarfarin.

MeasurementPrinciple:

OnevolumeofplasmaispredilutedwithfourvolumesofV-DEFPlasma.ThedilutionisthenincubatedwiththeAPTTreagentforastandardperiodoftime.CoagulationistriggeredbytheadditionofCaCl2intheabsenceandpresenceofexogenousAPCandthetimeforclotformationisrecorded.

ActivatedProteinC(APC)isaregulatorofthecoagulationcascade,byspecificallyinactivatingfactorsVaandVIIIa,inthepresenceofphospholipidsandcalcium. Inmostofthecases(morethan90%),ActivatedProteinCResistance(APCR)phenotypeiscausedbyaFactorVgenemutation(FactorVLeiden).Themutation,locatedonFactorVexon10(1691G–>A),ofargininetoglutamineonposition506,rendersFactorVaresistanttothecleavagebyActivatedProteinC.ThisgeneticanomalycanbeevidencedwithaclottingmethodperformedinthepresenceortheabsenceofActivatedProteinC.

KitComposition:

ReagentsandStABIlity:

1.V-DEFPlasma2vialsStabilized,lyophilizedhumanplasma,withalowleveloffactorVactivity,containingtheheparinantagoNISTPolybrene®.Reconstitutewith4.0mLofNCCLStypeIIwater.Allowtostandfor30minutesat20-25°C.Swirlgentlybeforeuse.

2.CaCl22vials2mLofcalciumchloride,0.025mol/L,inTrisbuffercontaining0.5%bovineserumalbumin.

3. APTTreagent2vials4mLofpurifiedphospholipidswithcolloidalsilicaascontactactivator.Containsapreservative.MixthoroughlyonaVortexmixerbeforeuse.

4.APC/CaCl22vialsHumanactivatedproteinCcolyophilizedwithCaCl2.Reconstitutewith2.0mLofNCCLStypeIIwater.Allowtostandfor30minutesat20-25°C.Swirlgentlybeforeuse.

5.ControlPlasmaLevel1.1vialLyophilizedhumanplasma.Reconstitutewith1.0mLofNCCLStypeIIwater.Allowtostandfor30minutesat20-25°C.Swirlgentlybeforeuse.

6.ControlPlasmaLevel2.1vialLyophilizedhumanplasma.Reconstitutewith1.0mLofNCCLStypeIIwater.Allowtostandfor30minutesat20-25°C.Swirlgentlybeforeuse.

 

TestingAlgorithm:

IDEALFOR:

  • Labslookingtobringtestingin-house
  • Labslookingforcostsavings
  • Orderingphysicianslookingforasensitiveassaywithrapidturnaround
  • Savesthelabfromhavingtoperformgenetictestonallpatients
  • Economicalalternativetogenetictesting

Background:

ActivatedProteinC(APC)isaregulatorofthecoagulationcascade,byspecificallyinactivatingfactorsVaandVIIIa,inthepresenceofphospholipidsandcalcium.

Inmostofthecases(morethan90%),ActivatedProteinCResistance(APCR)phenotypeiscausedbyaFactorVgenemutation(FactorVLeiden).Themutation,locatedonFactorVexon10(1691G–>A),ofargininetoglutamineonposition506,rendersFactorVaresistanttothecleavagebyActivatedProteinC.ThisgeneticanomalycanbeevidencedwithaclottingmethodperformedinthepresenceortheabsenceofActivatedProteinC.

ActivatedProteinCResistanceistestedbyusingaclottingmethodperformedwithorwithoutActivatedProteinC.

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品牌介绍
Diapharma使命宣言位于俄亥俄州西切斯特的Difarma Group,Inc.在诊断和研究领域销售止血、血栓形成、血小板功能测试、仪器和凋亡产品,并提供强大的技术能力和经验,以确保满足或超过客户的期望。地黄止血显色凝块酶联免疫吸附试验试剂盒历史1997年1月1日,由俄亥俄州富兰克林市的Pharmacia Hepar,Inc.成立,最初是Chromogenix基质和分析的独家美国和加拿大经销商。四分之一个多世纪前,Chromogenix开发了第一个显色底物技术,其前身是Kabi Diagnostica。卡比后来与法玛西亚合并。希帕玛目前的一些员工在法玛西亚肝素制造厂的显色部门工作。1998年,夏帕玛搬到了俄亥俄州的西切斯特,至今仍在那里。多年来,迪法玛扩大了其产品线,包括各种止血、细胞死亡、血小板功能、生态毒理学、化验、试剂、抗体和高级制造商的仪器。2017年,夏帕玛庆祝了20年的成功