Description:
ChromogenixCoamatic®FactorVIIIisachromogenicassaykitforthephotometricdeterminationoffactorVIIIactivityinplasma,suchaswhenidentifyingfactorVIIIdeficiencyormonitoringpatientsonreplacementtherapyaswellasforpotencyestimationofFVIIIconcentrates.This FactorVIIItestkitfulfillstherequirementsoftheEuropeanPharmacopoeiaforfactorVIIIconcentratetesting.Co-lyophilizationofreagentsforconvenienthandling.Reliabletoolforhemophiliaclassification.Nointerferencefromheparinlevelsupto1.5IU/ml.TheFactorVIIIkithastwomeasuringrangesplusvalidatedanddocumentedprotocolsforawiderangeofinstruments.
MeasurementPrinciple:
| FIXa,Ca2+,phospholipid | ||
| FactorX | → | FXa |
| FVIII | ||
| FXa | ||
| S-2765™ | → | Peptide+pNA |
Inthepresenceofcalciumionsandphospholipids,factorXisactivatedtofactorXabyfactorIXa.ThisactivationisgreatlystimulatedbyfactorVIIIwhichactsasacofactorinthisreaction.ByusingoptimalamountsofCa2+,phospholipidandfactorIXa,andanexcessoffactorX,therateofactivationoffactorXislinearlyrelatedtotheamountoffactorVIII.FactorXahydrolysesthechromogenicsubstrateS-2765™thusliberatingthechromophoricgroup,pNA.Thecolouristhenreadphotometricallyat405nm.ThegeneratedfactorXaandthustheintensityofcolourisproportionaltothefactorVIIIactivityinthesample.HydrolysisofS-2765™bythrombinformedispreventedbytheadditionofthesyntheticthrombininhibitorI-2581togetherwiththesubstrate.
Background:
PRECISE. SENSITIVE. ACCURATE. Coamatic®FactorVIIIforbetterhemophiliatesting.
TheChromogenixCoamatic®FactorVIIIassaykitprovidesreagentsandchromogenicmethodsfordeterminingfactorVIIIactivityinhumanplasmaandinfactorVIIIconcentrates.FactorVIIIisahighmolecularweightplasmaproteinwhichservesasacofactortofactorIXainitsactivationoffactorXtoXa.DeficiencyoffactorVIIIcausesaseverebleedingdisorder,hemophiliaA.TheseverityofthisbleedingdisorderisinverselyrelatedtothefactorVIIIconcentration.HemophiliaApatientsaregenerallyclassifiedaccordingtotheirfactorVIIIactivityintothreecategories:<0.01IU/mL=severe,0.01-0.04IU/mL=moderateand0.05-0.25IU/mL=mildhemophilia.
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