Description:
TheTECO®SerumCollagenIVELISAprovidesamethodforthequantitativedeterminationofcollagenIVinhumanserum.
KitComposition:
- CollagenIVAntibodycoatedMicroassayPlate12stripsof8wells(96breakapartwellsintotal),inaframe.ReadytoUse.
- CollagenIVCalibratorStockSolution,1000ug/L
- 10XWashBufferConcentrate
- DilutionBufferReadytoUse
- EnzymeConjugate:Anti-collagenIVmouseFab’conjugatedtoHRP,ReadytoUse
- TMBSubstrateReadytoUse
- StopSolution1MSulphuricAcid,ReadytoUse
- UncoatedMicroassayPlateReadytoUse
AssayPrinciple:
TheTECO®SerumCollagenIVELISAisdesignedfortheassayofserumcollagenIV.Itisasolidphaseone-stepsandwichELISA.CollagenIVinthesampleisboundsimultaneouslybyasolid-phasemonoclonalantibodyandamonoclonalantibody-enzymeconjugate,eachdirectedatdifferentantigenicsites.ThisresultsinthecollagenIVmoleculebeingsandwichedbetweenthesolidphaseandenzyme-labelledantibodies.Afterremovingunboundenzyme-labelledantibodyandsample,theplateisincubatedwithenzymesubstrate.TheresultantcolordevelopmentisdirectlyproportionaltotheamountofcollagenIVinthesample.
TypicalCalibrationCurve
TypicalcalibrationcurveobtainedusingSerumCollagenIVELISA.4-ParameterplotofA450/630nmversusug/LCollagenIV.Assayrangeis15.6-1000ug/LCollagenIV.
Background:
CollagenIVisatypeofnon-helicalformingcollagenfoundprimarilyisthebasallamina(basementmembrane).Chronicliverdiseasecomprisesanumberofprogressivedisorderswhichculminateinlivercirrhosisandwhicharecharacterizedbyexcessivedepositionofcollagen.
Althoughvarioustypesofcollagen(typeI,III,IV,VandVI)increaseintheliverwiththeprogressionoffibrosis,typeIVcollagen,aconstituentofthebasementmembrane,isparticularlynoteworthyforthefollowingreasons:itsserumlevelcorrelateswithhepaticlevelsofcollagenIV,serumlevelsofcollagenIVfallinresponsetoeffectivetherapy,anditistheearliesttypeofcollagentobesynthesizedduringexperimentalliverinjury.SerumcollagenIVlevelsareelevatedinavarietyofliverdiseases,andhavebeenfoundtobepredictiveoftherapyresponseinHepatitisCinfectionandtobesensitiveindicatorsoftherapyresponseinabstainingalcoholics.
